Review



pce2 ta blunt zero vector  (Vazyme Biotech Co)


Bioz Verified Symbol Vazyme Biotech Co is a verified supplier
Bioz Manufacturer Symbol Vazyme Biotech Co manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    Vazyme Biotech Co pce2 ta blunt zero vector
    Pce2 Ta Blunt Zero Vector, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 96/100, based on 290 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pce2+ta+blunt+zero+vector/5+min+TA+Blunt-Zero+Cloning+Kit/pmc13098232-284-6-9
    Average 96 stars, based on 290 article reviews
    pce2 ta blunt zero vector - by Bioz Stars, 2026-10
    96/100 stars

    Images

    Related Articles

    Polymerase Chain Reaction:

    Article Title: The leucokinin pathway regulates honey bee sugar consumption via Piezo.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Article Title: UHRF1 restricts HCoV-229E infection through epigenetic silencing of the viral receptor APN
    Article Snippet: Target fragments were amplified with the Methylation-specific PCR (MSP) Kit (Tiangen #EM101). .. PCR amplicons were cloned into the pCE2 TA/Blunt-Zero vector (Vazyme #C601-02), and eight colonies were selected for sequencing of CpG sites. .. DNA sequences of the APN proximal promoter underwent in silico bisulfite conversion using MethPrimer ( http://www.urogene.org/cgi-bin/methprimer2/MethSequence.cgi ).

    Article Title: Signaling Pathway Analysis and Downstream Genes Associated with Disease Resistance Mediated by GmSRC7
    Article Snippet: .. The PCR products were cloned into the pCE2-TA/Blunt-Zero vector (Vazyme, cat: C601) and verified by Sanger sequencing. ..

    Article Title: Signaling Pathway Analysis and Downstream Genes Associated with Disease Resistance Mediated by GmSRC7 .
    Article Snippet: .. The PCR products were cloned into the pCE2-TA/Blunt-Zero vector (Vazyme, cat: C601) and verified by Sanger sequencing. ..

    Clone Assay:

    Article Title: The leucokinin pathway regulates honey bee sugar consumption via Piezo.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Article Title: Repression of EEF1D by KSHV RTA promotes viral lytic reactivation
    Article Snippet: .. Amplified fragments were cloned into the pCE2 TA/Blunt-Zero vector (Vazyme, C601-01), transformed into E. coli DH5α, and positive clones were sequenced to assess their methylation status. .. ChIP assays were performed using the BeyoChIP Enzymatic ChIP Assay Kit (Beyotime, P2083S).

    Article Title: Repression of EEF1D by KSHV RTA promotes viral lytic reactivation.
    Article Snippet: .. Amplified fragments were cloned into the pCE2 TA/Blunt-Zero vector (Vazyme, C601-01), transformed into E. coli DH5α, and positive clones were sequenced to assess their methylation status. .. ChIP assays were performed using the BeyoChIP Enzymatic ChIP Assay Kit (Beyotime, P2083S).

    Article Title: UHRF1 restricts HCoV-229E infection through epigenetic silencing of the viral receptor APN
    Article Snippet: Target fragments were amplified with the Methylation-specific PCR (MSP) Kit (Tiangen #EM101). .. PCR amplicons were cloned into the pCE2 TA/Blunt-Zero vector (Vazyme #C601-02), and eight colonies were selected for sequencing of CpG sites. .. DNA sequences of the APN proximal promoter underwent in silico bisulfite conversion using MethPrimer ( http://www.urogene.org/cgi-bin/methprimer2/MethSequence.cgi ).

    Article Title: Signaling Pathway Analysis and Downstream Genes Associated with Disease Resistance Mediated by GmSRC7
    Article Snippet: .. The PCR products were cloned into the pCE2-TA/Blunt-Zero vector (Vazyme, cat: C601) and verified by Sanger sequencing. ..

    Article Title: Signaling Pathway Analysis and Downstream Genes Associated with Disease Resistance Mediated by GmSRC7 .
    Article Snippet: .. The PCR products were cloned into the pCE2-TA/Blunt-Zero vector (Vazyme, cat: C601) and verified by Sanger sequencing. ..

    Plasmid Preparation:

    Article Title: The leucokinin pathway regulates honey bee sugar consumption via Piezo.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Article Title: First identification of Sarcocystis medusiformis in Chinese sheep ( Ovis aries ): morphological and molecular characterization
    Article Snippet: Amplification was carried out in a Bio-Rad T100 thermal cycler (Bio-Rad Laboratories, Hercules, California, USA) under the following conditions: initial denaturation at 95 °C for 5 min; 35 cycles of 94 °C for 1 min, 53–57 °C (primer-specific) for 1 min, and 72 °C for 1 min; followed by a final extension at 72 °C for 10 min. PCR products were purified using the E.Z.N.A. .. ® Gel Extraction Kit (Omega Bio-Tek, Inc., USA), ligated into the pCE2 TA/Blunt-Zero vector (5 min TA/Blunt-Zero Cloning Kit, Vazyme Biotech Co., Ltd., Nanjing, China), and transformed into TreliefTM 5α Chemically Competent Cells (Tsingke Biotechnology Co., Ltd., Beijing, China). .. Positive clones were sequenced bidirectionally on an ABI PRISMTM 3730 XL DNA Analyzer (Applied Biosystems, Foster, USA).

    Article Title: Repression of EEF1D by KSHV RTA promotes viral lytic reactivation
    Article Snippet: .. Amplified fragments were cloned into the pCE2 TA/Blunt-Zero vector (Vazyme, C601-01), transformed into E. coli DH5α, and positive clones were sequenced to assess their methylation status. .. ChIP assays were performed using the BeyoChIP Enzymatic ChIP Assay Kit (Beyotime, P2083S).

    Article Title: Repression of EEF1D by KSHV RTA promotes viral lytic reactivation.
    Article Snippet: .. Amplified fragments were cloned into the pCE2 TA/Blunt-Zero vector (Vazyme, C601-01), transformed into E. coli DH5α, and positive clones were sequenced to assess their methylation status. .. ChIP assays were performed using the BeyoChIP Enzymatic ChIP Assay Kit (Beyotime, P2083S).

    Article Title: UHRF1 restricts HCoV-229E infection through epigenetic silencing of the viral receptor APN
    Article Snippet: Target fragments were amplified with the Methylation-specific PCR (MSP) Kit (Tiangen #EM101). .. PCR amplicons were cloned into the pCE2 TA/Blunt-Zero vector (Vazyme #C601-02), and eight colonies were selected for sequencing of CpG sites. .. DNA sequences of the APN proximal promoter underwent in silico bisulfite conversion using MethPrimer ( http://www.urogene.org/cgi-bin/methprimer2/MethSequence.cgi ).

    Article Title: Signaling Pathway Analysis and Downstream Genes Associated with Disease Resistance Mediated by GmSRC7
    Article Snippet: .. The PCR products were cloned into the pCE2-TA/Blunt-Zero vector (Vazyme, cat: C601) and verified by Sanger sequencing. ..

    Article Title: Signaling Pathway Analysis and Downstream Genes Associated with Disease Resistance Mediated by GmSRC7 .
    Article Snippet: .. The PCR products were cloned into the pCE2-TA/Blunt-Zero vector (Vazyme, cat: C601) and verified by Sanger sequencing. ..

    Gel Extraction:

    Article Title: First identification of Sarcocystis medusiformis in Chinese sheep ( Ovis aries ): morphological and molecular characterization
    Article Snippet: Amplification was carried out in a Bio-Rad T100 thermal cycler (Bio-Rad Laboratories, Hercules, California, USA) under the following conditions: initial denaturation at 95 °C for 5 min; 35 cycles of 94 °C for 1 min, 53–57 °C (primer-specific) for 1 min, and 72 °C for 1 min; followed by a final extension at 72 °C for 10 min. PCR products were purified using the E.Z.N.A. .. ® Gel Extraction Kit (Omega Bio-Tek, Inc., USA), ligated into the pCE2 TA/Blunt-Zero vector (5 min TA/Blunt-Zero Cloning Kit, Vazyme Biotech Co., Ltd., Nanjing, China), and transformed into TreliefTM 5α Chemically Competent Cells (Tsingke Biotechnology Co., Ltd., Beijing, China). .. Positive clones were sequenced bidirectionally on an ABI PRISMTM 3730 XL DNA Analyzer (Applied Biosystems, Foster, USA).

    Cloning:

    Article Title: First identification of Sarcocystis medusiformis in Chinese sheep ( Ovis aries ): morphological and molecular characterization
    Article Snippet: Amplification was carried out in a Bio-Rad T100 thermal cycler (Bio-Rad Laboratories, Hercules, California, USA) under the following conditions: initial denaturation at 95 °C for 5 min; 35 cycles of 94 °C for 1 min, 53–57 °C (primer-specific) for 1 min, and 72 °C for 1 min; followed by a final extension at 72 °C for 10 min. PCR products were purified using the E.Z.N.A. .. ® Gel Extraction Kit (Omega Bio-Tek, Inc., USA), ligated into the pCE2 TA/Blunt-Zero vector (5 min TA/Blunt-Zero Cloning Kit, Vazyme Biotech Co., Ltd., Nanjing, China), and transformed into TreliefTM 5α Chemically Competent Cells (Tsingke Biotechnology Co., Ltd., Beijing, China). .. Positive clones were sequenced bidirectionally on an ABI PRISMTM 3730 XL DNA Analyzer (Applied Biosystems, Foster, USA).

    Transformation Assay:

    Article Title: First identification of Sarcocystis medusiformis in Chinese sheep ( Ovis aries ): morphological and molecular characterization
    Article Snippet: Amplification was carried out in a Bio-Rad T100 thermal cycler (Bio-Rad Laboratories, Hercules, California, USA) under the following conditions: initial denaturation at 95 °C for 5 min; 35 cycles of 94 °C for 1 min, 53–57 °C (primer-specific) for 1 min, and 72 °C for 1 min; followed by a final extension at 72 °C for 10 min. PCR products were purified using the E.Z.N.A. .. ® Gel Extraction Kit (Omega Bio-Tek, Inc., USA), ligated into the pCE2 TA/Blunt-Zero vector (5 min TA/Blunt-Zero Cloning Kit, Vazyme Biotech Co., Ltd., Nanjing, China), and transformed into TreliefTM 5α Chemically Competent Cells (Tsingke Biotechnology Co., Ltd., Beijing, China). .. Positive clones were sequenced bidirectionally on an ABI PRISMTM 3730 XL DNA Analyzer (Applied Biosystems, Foster, USA).

    Article Title: Repression of EEF1D by KSHV RTA promotes viral lytic reactivation
    Article Snippet: .. Amplified fragments were cloned into the pCE2 TA/Blunt-Zero vector (Vazyme, C601-01), transformed into E. coli DH5α, and positive clones were sequenced to assess their methylation status. .. ChIP assays were performed using the BeyoChIP Enzymatic ChIP Assay Kit (Beyotime, P2083S).

    Article Title: Repression of EEF1D by KSHV RTA promotes viral lytic reactivation.
    Article Snippet: .. Amplified fragments were cloned into the pCE2 TA/Blunt-Zero vector (Vazyme, C601-01), transformed into E. coli DH5α, and positive clones were sequenced to assess their methylation status. .. ChIP assays were performed using the BeyoChIP Enzymatic ChIP Assay Kit (Beyotime, P2083S).

    Amplification:

    Article Title: Repression of EEF1D by KSHV RTA promotes viral lytic reactivation
    Article Snippet: .. Amplified fragments were cloned into the pCE2 TA/Blunt-Zero vector (Vazyme, C601-01), transformed into E. coli DH5α, and positive clones were sequenced to assess their methylation status. .. ChIP assays were performed using the BeyoChIP Enzymatic ChIP Assay Kit (Beyotime, P2083S).

    Article Title: Repression of EEF1D by KSHV RTA promotes viral lytic reactivation.
    Article Snippet: .. Amplified fragments were cloned into the pCE2 TA/Blunt-Zero vector (Vazyme, C601-01), transformed into E. coli DH5α, and positive clones were sequenced to assess their methylation status. .. ChIP assays were performed using the BeyoChIP Enzymatic ChIP Assay Kit (Beyotime, P2083S).

    Methylation:

    Article Title: Repression of EEF1D by KSHV RTA promotes viral lytic reactivation
    Article Snippet: .. Amplified fragments were cloned into the pCE2 TA/Blunt-Zero vector (Vazyme, C601-01), transformed into E. coli DH5α, and positive clones were sequenced to assess their methylation status. .. ChIP assays were performed using the BeyoChIP Enzymatic ChIP Assay Kit (Beyotime, P2083S).

    Article Title: Repression of EEF1D by KSHV RTA promotes viral lytic reactivation.
    Article Snippet: .. Amplified fragments were cloned into the pCE2 TA/Blunt-Zero vector (Vazyme, C601-01), transformed into E. coli DH5α, and positive clones were sequenced to assess their methylation status. .. ChIP assays were performed using the BeyoChIP Enzymatic ChIP Assay Kit (Beyotime, P2083S).

    Sequencing:

    Article Title: UHRF1 restricts HCoV-229E infection through epigenetic silencing of the viral receptor APN
    Article Snippet: Target fragments were amplified with the Methylation-specific PCR (MSP) Kit (Tiangen #EM101). .. PCR amplicons were cloned into the pCE2 TA/Blunt-Zero vector (Vazyme #C601-02), and eight colonies were selected for sequencing of CpG sites. .. DNA sequences of the APN proximal promoter underwent in silico bisulfite conversion using MethPrimer ( http://www.urogene.org/cgi-bin/methprimer2/MethSequence.cgi ).

    Article Title: Signaling Pathway Analysis and Downstream Genes Associated with Disease Resistance Mediated by GmSRC7
    Article Snippet: .. The PCR products were cloned into the pCE2-TA/Blunt-Zero vector (Vazyme, cat: C601) and verified by Sanger sequencing. ..

    Article Title: Signaling Pathway Analysis and Downstream Genes Associated with Disease Resistance Mediated by GmSRC7 .
    Article Snippet: .. The PCR products were cloned into the pCE2-TA/Blunt-Zero vector (Vazyme, cat: C601) and verified by Sanger sequencing. ..



    Similar Products

    96
    Vazyme Biotech Co pce2 ta blunt zero vector
    Pce2 Ta Blunt Zero Vector, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pce2+ta+blunt+zero+vector/5+min+TA+Blunt-Zero+Cloning+Kit/pmc13098232-284-6-9
    Average 96 stars, based on 1 article reviews
    pce2 ta blunt zero vector - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    Image Search Results